Journal: Kidney Research and Clinical Practice

Article Title: Chronic renal failure induces cell death in rat hippocampal CA1 via upregulation of αCaMKII/NR2A synaptic complex and phosphorylated GluR1-containing AMPA receptor cascades

doi: 10.1016/j.krcp.2014.07.003

Figure Lengend Snippet: Determination of the phosphorylations of GluR1 at ser831 or ser845 in the hippocampal CA1 subfield after CRF. (A) Samples of sham-operated control and CRF CA1 tissues were immunoblotted with anti-GluR1-ser831 or anti-GluR1-ser845. Immunoblotting for GluR1-ser831 and GluR1-ser845 revealed major strong bands at 132 kDa and 100 kDa, respectively. (B) Densitometric analyses revealed that CRF significantly increased in p-GluR1 (ser831), but not p-GluR1 (ser845), in the CA1 subfield. Results are means±SEMs. Control n =8, CRF n =8. * P <0.01. CA1, cornu ammonis 1; CRF, chronic renal failure; GluR1, glutamate receptor type 1; SEM, standard error of mean.

Article Snippet: Membranes were then blocked with 5% milk in PBS-T (80mM Na 2 HPO 4 , 20mM NaH 2 PO 4 , 100mM NaCl, 0.1% Tween 20; pH 7.5) for 1 hour and incubated overnight at 4°C with mouse antineuronal nuclei (NeuN; 1:1,000; Chemicon, Temecula, CA, USA), rabbit antiPSD-95 (1:1,000), mouse anti-CaMKII (1:2,000), or mouse anti-p-Ser831 and 845 GluR1 (1:1,000; Santa Cruz Biotechnology, Texas, USA).

Techniques: Control, Western Blot